| Autor: |
Rajapandi, T, Dolan, K M, Oliver, D B |
| Zdroj: |
Journal of Bacteriology; November 1991, Vol. 173 Issue: 22 p7092-7097, 6p |
| Abstrakt: |
TnphoA insertions in the first gene of the Escherichia coli secA operon, gene X, were isolated and analyzed. Studies of the Gene X-PhoA fusion proteins showed that gene X encodes a secretory protein, since the fusion proteins possessed normal alkaline phosphatase activity and a substantial portion of this activity was found in the periplasm. In addition, the Gene X-PhoA fusion proteins were initially synthesized with a cleavable signal peptide. A gene X::TnphoA insertion was used to construct a strain containing a disrupted chromosomal copy of gene X. Analysis of this strain indicated that gene X is nonessential for cell growth and viability and does not appear to play an essential role in the process of protein export. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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