| Autor: |
Wei, X A, Pelcher, L E, Rank, G H |
| Zdroj: |
Journal of Bacteriology; February 1991, Vol. 173 Issue: 3 p1181-1186, 6p |
| Abstrakt: |
2 microns DNA isolated from industrial Saccharomyces cerevisiae yeasts exhibited extensive restriction fragment length polymorphisms. At least five 2 microns species were identified from eleven [cir+] strains. Southern hybridization mapped restriction fragment length polymorphisms at STB, a cis-acting locus essential for plasmid partitioning. Some 2 microns variants (e.g., 4110-2 microns and 4108-2 microns) had an altered number of 125-bp consensus repeats at STB. However, the corresponding region of 7754-2 microns has only approximately 70% nucleotide sequence homology with the 125-bp STB consensus repeat. YRp plasmids containing 7754-2 microns STB behave as YEp plasmids in laboratory yeasts, thereby indicating STB sequence divergence coupled to conservation of function. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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