Autor: |
Vijayakumar, M N, Morrison, D A |
Zdroj: |
Journal of Bacteriology; November 1983, Vol. 156 Issue: 2 p644-648, 5p |
Abstrakt: |
Uptake of DNA and genetic recombination proceeded normally in competent Streptococcus pneumoniae despite inhibition of DNA replication by 6-(p-hydroxyphenylazo)-uracil. Immediately after a brief uptake period, 68% of donor DNA label was in eclipse complex form, and 22% was in low-molecular-weight products; by the completion of integration at 10 min, 23% was integrated into the chromosome, and the rest was lost from the cell. Throughout the process, less than 1% was found as free single strands. The DNA in eclipse complex is therefore an intermediate in the integration process. |
Databáze: |
Supplemental Index |
Externí odkaz: |
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