Fate of DNA in eclipse complex during genetic transformation in Streptococcus pneumoniae

Autor: Vijayakumar, M N, Morrison, D A
Zdroj: Journal of Bacteriology; November 1983, Vol. 156 Issue: 2 p644-648, 5p
Abstrakt: Uptake of DNA and genetic recombination proceeded normally in competent Streptococcus pneumoniae despite inhibition of DNA replication by 6-(p-hydroxyphenylazo)-uracil. Immediately after a brief uptake period, 68% of donor DNA label was in eclipse complex form, and 22% was in low-molecular-weight products; by the completion of integration at 10 min, 23% was integrated into the chromosome, and the rest was lost from the cell. Throughout the process, less than 1% was found as free single strands. The DNA in eclipse complex is therefore an intermediate in the integration process.
Databáze: Supplemental Index