Phycocyanin synthesis and degradation in the blue-green bacterium Anacystis nidulans

Autor: Lau, R H, MacKenzie, M M, Doolittle, W F
Zdroj: Journal of Bacteriology; December 1977, Vol. 132 Issue: 3 p771-778, 8p
Abstrakt: Cellular content and rates of synthesis of the apoprotein subunits of phycocyanin in Anacystis nidulans cultures undergoing, and recovering from, nitrate starvation were measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total and immunoprecipitable soluble proteins. Results indicated that (i) nitrate starvation provokes coordinate degradation of apoprotein subunits: (ii) de novo synthesis of these subunits is selectively depressed during starvation; (iii) nitrate restoration provokes coordinate increases in the rates of synthesis of these subunits, although maximal rates are not achieved for 6 to 10 h after readdition of nitrate; and (iv) illumination affects both relative and absolute rates of apoprotein formation.
Databáze: Supplemental Index