| Autor: |
McCollister, Bruce D., Myers, Jesse T., Jones-Carson, Jessica, Voelker, Dennis R., Vázquez-Torres, Andrés |
| Zdroj: |
Infection and Immunity; November 2007, Vol. 75 Issue: 11 p5346-5352, 7p |
| Abstrakt: |
ABSTRACTWe have identified acid sphingomyelinase (ASM) as an important player in the early and late anti-Salmonellaactivity of macrophages. A functional ASM participated in the killing activity of macrophages against wild-type Salmonella entericaserovar Typhimurium. The role of ASM in early macrophage killing of Salmonellaappears to be linked to an active NADPH phagocyte oxidase enzymatic complex, since the flavoprotein inhibitor diphenyleneiodonium not only blocked a productive respiratory burst but also abrogated the survival advantage of Salmonellain macrophages lacking ASM. Lack of ASM activity also increased the intracellular survival of an isogenic ΔspiC::FRT Salmonellastrain deficient in a translocator and effector of the Salmonellapathogenicity island 2 (SPI2) type III secretion system, suggesting that the antimicrobial activity associated with ASM is manifested regardless of the SPI2 status of the bacteria. Constitutively expressed ASM is responsible for the role that this lipid-metabolizing hydrolase plays in the innate host defense of macrophages against Salmonella. Accordingly, the ASM activity and intracellular concentration and composition of ceramide, gangliosides, and neutral sphingolipids did not increase upon Salmonellainfection. Salmonellatriggered, nonetheless, a significant increase in the secreted fraction of ASM. Collectively, these findings have elucidated a novel role for constitutive ASM in the anti-Salmonellaactivity of murine macrophages. |
| Databáze: |
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