Biosynthesis of Arginine in L Cells Infected with Chlamydiae

Autor: Treuhaft, Mary W., Moulder, James W.
Zdroj: Journal of Bacteriology; December 1968, Vol. 96 Issue: 6 p2004-2011, 8p
Abstrakt: Three members of the genus Chlamydiawere examined for their ability to synthesize arginine, an ability their L cell (mouse fibroblasts) hosts lacked. C. psittaci(strain 6BC) multiplied in arginine-free medium 199 without significant decrease in titer and incroporated 14C-glutamate and 14C-ornithine into the arginine fraction of its protein. In arginine-free media, C. trachomatis(strain mouse pneumonitis) and C. psittaci(strain meningopneumonitis) grew to only 1 to 10% of the titer obtained in arginine-containing media. The decreased ability of these two strains to multiply in arginine-free media was paralleled by a decreased ability of infected host cells to incorporate 14C-glutamate into protein arginine. These results suggest that chlamydiae either synthesize arginine themselves, or, in some unknown manner, cause their host cells to do so.
Databáze: Supplemental Index