| Autor: |
McRipley, R. J., Sbarra, A. J. |
| Zdroj: |
Journal of Bacteriology; November 1967, Vol. 94 Issue: 5 p1425-1430, 6p |
| Abstrakt: |
An antimicrobial system in polymorphonuclear neutrophils (PMN) consisting of myeloperoxidase and hydrogen peroxide has been proposed. This system appears to be activated during phagocytosis as a result of the stimulated metabolic activities. A lysed-granules (LG) fraction was prepared from guinea pig exudative PMN. LG alone possessed bactericidal activity which was related to the pH of the reaction; the lower the pH, the more marked the activity. When low concentrations of both H2O2and LG were combined under conditions where neither factor alone exhibited significant killing power, there was a striking increase in bactericidal activity. This enhanced activity was much greater than an additive effect. The LG-peroxide antibacterial system was most active over a pH range of 4.0 to 6.0. The activity of the LG-peroxide system was essentially abolished by peroxidase inhibitors, NaN3, KCN, and aminotriazole. The antibacterial activity of this system was nonspecific in nature, being equally effective against gram-negative and gram-positive organisms. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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