| Autor: |
Mve-Obiang, Armand, Lee, Richard E., Umstot, Edward S., Trott, Kristin A., Grammer, Timothy C., Parker, John M., Ranger, Brian S., Grainger, Robert, Mahrous, Engu A., Small, P. L. C. |
| Zdroj: |
Infection and Immunity; June 2005, Vol. 73 Issue: 6 p3307-3312, 6p |
| Abstrakt: |
ABSTRACTMycobacterium ulcerans, the causative agent of Buruli ulcer, produces a macrolide toxin, mycolactone A/B, which is thought to play a major role in virulence. A disease similar to Buruli ulcer recently appeared in United States frog colonies following importation of the West African frog, Xenopus tropicalis. The taxonomic position of the frog pathogen has not been fully elucidated, but this organism, tentatively designated Mycobacterium liflandii, is closely related to M. ulceransand Mycobacterium marinum, and as further evidence is gathered, it will most likely be considered a subspecies of one of these species. In this paper we show that M. liflandiiproduces a novel plasmid-encoded mycolactone, mycolactone E. M. liflandiicontains all of the genes in the mycolactone cluster with the exception of that encoding CYP140A2, a putative p450 monooxygenase. Although the core lactone structure is conserved in mycolactone E, the fatty acid side chain differs from that of mycolactone A/B in the number of hydroxyl groups and double bonds. The cytopathic phenotype of mycolactone E is identical to that of mycolactone A/B, although it is less potent. To further characterize the relationship between M. liflandiiand M. ulcerans, strains were analyzed for the presence of the RD1 region genes, esxA(ESAT-6) and esxB(CFP-10). The M. ulceransgenome strain has a deletion in RD1 and lacks these genes. The results of these studies show that M. liflandiicontains both esxAand esxB. |
| Databáze: |
Supplemental Index |
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