| Autor: |
Ugrinovic, Sanja, Ménager, Nathalie, Goh, Natalie, Mastroeni, Pietro |
| Zdroj: |
Infection and Immunity; December 2003, Vol. 71 Issue: 12 p6808-6819, 12p |
| Abstrakt: |
ABSTRACTInfection of mice with Salmonella entericaserovar Typhimurium induces strong Th1 T-cell responses that are central to the control of the infection. In the present study, we examined the role of B cells in the development of Th1 T-cell responses to Salmonellaby using gene-targeted B-cell-deficient mice (Igh-6−/−mice). The development of Th1 T-cell responses in Igh-6−/−mice was impaired in the early stage of a primary infection. This impairment persisted throughout the course of the disease. The ability of T cells to produce the Th1 cytokine gamma interferon and the frequency at which they did so were lower in Igh-6−/−mice than in control mice. We also observed a transient switch toward Th2 cytokine production in Igh-6−/−mice. Thus, B cells are important for the induction of protective Th1 T-cell responses in the early phase of a Salmonellainfection. Activated B cells express high levels of major histocompatibility complex and costimulatory molecules and are nearly as effective as dendritic cells in their antigen-presenting cell (APC) activity. However, their importance as APCs in infection and their role in initiating and/or maintaining T-cell responses are unknown. Here, we show that B cells upregulate costimulatory molecules upon in vitro stimulation with S. entericaserovar Typhimurium and that they can present Salmonellaantigens to Salmonella-specific CD4+T cells. Our results show that B cells are important for the development of T-cell responses in the early stage of a Salmonellainfection and that this property may be due to their ability to present antigens to T cells. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
