| Autor: |
Capozzo, Alejandra V. E., Creydt, Virginia Pistone, Dran, Graciela, Fernández, Gabriela, Gómez, Sonia, Bentancor, Leticia V., Rubel, Carolina, Ibarra, Cristina, Isturiz, Martín, Palermo, Marina S. |
| Zdroj: |
Infection and Immunity; July 2003, Vol. 71 Issue: 7 p3971-3978, 8p |
| Abstrakt: |
ABSTRACTShiga toxin type 2 (Stx2) produced by Escherichia coliO:157H7 can cause hemolytic-uremic syndrome in children, a disease for which there is neither a vaccine nor an effective treatment. This toxin consists of an enzymatically active A subunit and a pentameric B subunit responsible for the toxin binding to host cells, and also found to be immunogenic in rabbits. In this study we developed eukaryotic plasmids expressing the B subunit gene of Stx2 (pStx2B) and the B subunit plus the gene coding for the A subunit with an active-site deletion (pStx2ΔA). Transfection of eukaryotic cells with these plasmids produced proteins of the expected molecular weight which reacted with specific monoclonal antibodies. Newborn and adult BALB/c mice immunized with two intramuscular injections of each plasmid, either alone or together with the same vector expressing the granulocyte and monocyte colony-stimulating factor (pGM-CSF), elicited a specific Th1-biased humoral response. The effect of pGM-CSF as an adjuvant plasmid was particularly notable in newborn mice and in pStx2B-vaccinated adult mice. Stx2-neutralizing activity, evaluated in vitro on VERO cell monolayers, correlated with in vivo protection. This is the first report using plasmids to induce a neutralizing humoral immune response against the Stx2. |
| Databáze: |
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