| Autor: |
Gordhan, Bhavna G., Smith, Debbie A., Alderton, Heidi, McAdam, Ruth A., Bancroft, Gregory J., Mizrahi, Valerie |
| Zdroj: |
Infection and Immunity; June 2002, Vol. 70 Issue: 6 p3080-3084, 5p |
| Abstrakt: |
ABSTRACTA mutant of Mycobacterium tuberculosisdefective in the metabolism of l-arginine was constructed by allelic exchange mutagenesis. The argFmutant strain required exogenous l-arginine for growth in vitro, and in the presence of 0.96 mM l-arginine, it achieved a growth rate and cell density in stationary phase comparable to those of the wild type. The mutant strain was also able to grow in the presence of high concentrations of argininosuccinate, but its auxotrophic phenotype could not be rescued by l-citrulline, suggesting that the ΔargF::hygmutation exerted a polar effect on the downstream argGgene but not on argH. The mutant strain displayed reduced virulence in immunodeficient SCID mice and was highly attenuated in immunocompetent DBA/2 mice, suggesting that l-arginine availability is restricted in vivo. |
| Databáze: |
Supplemental Index |
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