| Autor: |
Hickey, Thomas E., McVeigh, Annette L., Scott, Daniel A., Michielutti, Ronda E., Bixby, Alyssa, Carroll, Shannon A., Bourgeois, A. Louis, Guerry, Patricia |
| Zdroj: |
Infection and Immunity; December 2000, Vol. 68 Issue: 12 p6535-6541, 7p |
| Abstrakt: |
ABSTRACTLive cells of Campylobacter jejuniandCampylobacter colican induce release of interleukin-8 (IL-8) from INT407 cells. Additionally, membrane fractions of C. jejuni81-176, but not membrane fractions of C. colistrains, can also induce release of IL-8. Membrane preparations from 81-176 mutants defective in any of the three membrane-associated protein subunits of cytolethal distending toxin (CDT) were unable to induce IL-8. The presence of the three cdtgenes on a shuttle plasmid in transrestored both CDT activity and the ability to release IL-8 to membrane fractions. However, CDT mutations did not affect the ability of 81-176 to induce IL-8 during adherence to or invasion of INT407 cells. When C. jejuni cdtgenes were transferred on a shuttle plasmid into a C. colistrain lacking CDT, membrane preparations became positive in both CDT and IL-8 assays. Growth of C. jejuniin physiological levels of sodium deoxycholate released all three CDT proteins, as well as CDT activity and IL-8 activity, from membranes into supernatants. Antibodies against recombinant forms of each of the three CDT subunit proteins neutralized both CDT activity and the activity responsible for IL-8 release. The data suggest that C. jejunican induce IL-8 release from INT407 cells by two independent mechanisms, one of which requires adherence and/or invasion and the second of which requires CDT. |
| Databáze: |
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