| Autor: |
Joshi, Sunil K., Bharadwaj, Ashima, Chatterjee, Shyama, Chauhan, V. S. |
| Zdroj: |
Infection and Immunity; January 2000, Vol. 68 Issue: 1 p141-150, 10p |
| Abstrakt: |
ABSTRACTLiver-stage antigen 1 (LSA-1) is a potential vaccine candidate against preerythrocytic stages of malaria. We report here the immunogenicity of linear synthetic constructs delineated as TH-cell determinants from the nonrepeat regions ofPlasmodium falciparumLSA-1 in murine models and human subjects from areas where malaria is endemic in Rajasthan State, India. Seven peptide constructs (LS1.1 to LS1.7) corresponding to predicted T-cell sites from both the N- and C-terminal regions and peptide LS1R from a repeat region of PfLSA-1 were synthesized to analyze the cellular immune responses. These linear peptides were also tested for humoral responses in order to determine if there were any overlapping B-cell epitopes in the predicted T-cell sites. Most peptides induced cellular responses in peptide-immunized BALB/c and C57BL/6 mice as measured by proliferation and cytokine analysis. Cross-reactive T-cell recognition of P. falciparum-based peptides in Plasmodium berghei-immune animals was evaluated, but only one peptide, LS1.2 (amino acids 1742 to 1760) triggered T-cell proliferation and interleukin-2 and gamma interferon secretion in P. berghei-immune splenocytes of BALB/c and C57BL/6 mice as well as in Thamnomys gazellae(natural host of P. bergheiANKA). In an enzyme-linked immunosorbent assay with the peptides, only one peptide, LS1.1, was recognized by anti-P. bergheiliver-stage serum. Three peptides (LS1.1, LS1.2, and LS1.3) of the eight peptides tested in this study were recognized by a relatively large percentage ofP. falciparum-exposed human subjects; the reactivities ranged from ∼45% for LS1.3 to ∼60% for LS1.1 and LS1.2. Interestingly, all of the eight putative T-cell determinants were also recognized by the sera collected from malaria patients, although the response was variable in nature. These TH- and B-cell epitopes may be of potential value for preerythrocytic antigen-based malaria subunit vaccine formulations. |
| Databáze: |
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