| Autor: |
Weldingh, Karin, Rosenkrands, Ida, Jacobsen, Susanne, Rasmussen, Peter Birk, Elhay, Martin J., Andersen, Peter |
| Zdroj: |
Infection and Immunity; August 1998, Vol. 66 Issue: 8 p3492-3500, 9p |
| Abstrakt: |
ABSTRACTCulture filtrate from Mycobacterium tuberculosiscontains molecules which promote high levels of protective immunity in animal models of subunit vaccination against tuberculosis. We have used two-dimensional electrophoresis for analysis and purification of six novel M. tuberculosisculture filtrate proteins (CFPs): CFP17, CFP20, CFP21, CFP22, CFP25, and CFP28. The proteins were tested for recognition by M. tuberculosis-reactive memory cells from different strains of inbred mice and for their capacity to induce a skin test response in M. tuberculosis-infected guinea pigs. CFP17, CFP20, CFP21 and CFP25 induced both a high gamma interferon release and a strong delayed-type hypersensitivity response, and CFP21 was broadly recognized by different strains of inbred mice. N-terminal sequences were obtained for the six proteins, and the corresponding genes were identified in the Sanger M. tuberculosisgenome database. In parallel we established a two-dimensional electrophoresis reference map of short-term culture filtrate components and mapped novel proteins as well as already-known CFP. |
| Databáze: |
Supplemental Index |
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