| Autor: |
Djoba Siawaya, Joel Fleury, Bapela, Nchinya Bennedict, Ronacher, Katharina, Beyers, Nulda, van Helden, Paul, Walzl, Gerhard |
| Zdroj: |
Clinical and Vaccine Immunology (formerly CDLI); August 2008, Vol. 15 Issue: 8 p1165-1170, 6p |
| Abstrakt: |
ABSTRACTThis study investigated interleukin-4 (IL-4), IL-4d2, transforming growth factor beta (TGF-ß), TGF-ßRII, Foxp3, GATA-3, T-bet, and gamma interferon (IFN-?) transcription in peripheral blood samples of adult pulmonary tuberculosis patients prior to and after 1 week of therapy. Twenty patients with positive results for sputum culture for Mycobacterium tuberculosiswere enrolled and treated with directly observed short-course antituberculosis chemotherapy. Early treatment response was assessed. At the end of the intensive phase of treatment (month 2), 12 patients remained sputum culture positive (slow responders) and 8 converted to a negative culture (fast responders). Only the expression levels of IL-4 (4-fold decrease) and IL-4d2 (32-fold increase) changed significantly during the first week of therapy in the 20 patients. No baseline differences were present between the responder groups, but fast responders had significantly higher IL-4 transcripts than slow responders at week 1. Fast responders showed a 19-fold upregulation and slow responders a 47-fold upregulation of IL-4d2 at week 1. Only slow responders also showed a significant decrease in IL-4 expression at week 1. There were no significant differences in expression of TGF-ß, TGF-ßRII, Foxp3, IFN-?, and GATA-3 between the groups. These data show that differential IL-4-related gene expression in the early stage of antituberculosis treatment accompanies differential treatment responses and may hold promise as a marker for treatment effect. |
| Databáze: |
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