| Autor: |
Baszler, Timothy V., Shkap, Varda, Mwangi, Waithaka, Davies, Christopher J., Mathison, Bruce A., Mazuz, Monica, Resnikov, Dror, Fish, Lea, Leibovitch, Benjamin, Staska, Lauren M., Savitsky, Igor |
| Zdroj: |
Clinical and Vaccine Immunology (formerly CDLI); April 2008, Vol. 15 Issue: 4 p659-667, 9p |
| Abstrakt: |
ABSTRACTInfection of cattle with Neospora caninumprotozoa, the causative agent of bovine protozoal abortion, results in robust cellular and humoral immune responses, particularly CD4+T-lymphocyte activation and gamma interferon (IFN-?) secretion. In the present study, N. caninumSRS2 (NcSRS2) T-lymphocyte-epitope-bearing subunits were incorporated into DNA and peptide preparations to assess CD4+cell proliferation and IFN-? T-lymphocyte-secretion immune responses in cattle with predetermined major histocompatibility complex (MHC) genotypes. In order to optimize dendritic-cell processing, NcSRS2 DNA vaccine was delivered with granulocyte macrophage-colony-stimulating factor and Flt3 ligand adjuvant. The synthesized NcSRS2 peptides were coupled with a palmitic acid molecule (lipopeptide) and delivered with Freund's adjuvant. Cattle vaccinated with NcSRS2 DNA vaccine alone did not induce T-lymphocyte activation or IFN-? secretion, whereas subsequent booster inoculation with NcSRS2-lipopeptides induced robust NcSRS2-specific immune responses. Compared to the response in control animals, NcSRS2-lipopeptide-immunized cattle had significantly increased NcSRS2-specific T-lymphocyte proliferation, numbers of IFN-?-secreting peripheral blood mononuclear cells, and immunoglobulin G1 (IgG1) and IgG2a antibody levels. The findings show that N. caninumNcSRS2 subunits bearing T-lymphocyte epitopes induced cell-mediated immune responses similar to the protective immune responses previously described against live parasite infection, namely T-lymphocyte activation and IFN-? secretion. The findings support the investigation of NcSRS2 immunogens for protection against N. caninum-induced fetal infection and abortion in cattle. |
| Databáze: |
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