Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays

Autor: Vela´zquez, Blanca, Massaldi, Hugo, Battistoni, Julio, Chabalgoity, Jose´ A.
Zdroj: Clinical and Vaccine Immunology (formerly CDLI); May 2005, Vol. 12 Issue: 5 p683-684, 2p
Abstrakt: ABSTRACTCommercially available immunoassays for assessment of anti-streptolysin-O antibodies use native streptolysin-O obtained by a complex process. We prepared a biologically active recombinant streptolysin-O with higher yield and a simpler purification process. An enzyme-linked immunosorbent assay developed with this recombinant showed good correlation with a commercial test, suggesting that it could be suitable for immunoassays.
Databáze: Supplemental Index