| Autor: |
Yoneda, Masahiro, Hirofuji, Takao, Motooka, Noriko, Nozoe, Koji, Shigenaga, Kayoko, Anan, Hisashi, Miura, Mayumi, Kabashima, Hiroaki, Matsumoto, Akiko, Maeda, Katsumasa |
| Zdroj: |
Clinical and Vaccine Immunology (formerly CDLI); May 2003, Vol. 10 Issue: 3 p383-387, 5p |
| Abstrakt: |
ABSTRACTBacteroides forsythusis one of the important periodontopathic bacteria, and this microorganism is known to have an S-layer outside the outer membrane. The S-layer-like antigens were recently isolated from B. forsythus, and they were found to be 270- and 230-kDa proteins in the envelope fraction. In this study, these proteins were confirmed to be specific to B. forsythusby sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and they were clearly recognized by sera from patients with adult and early-onset periodontitis in Western immmunoblot analysis. We compared the immunoglobulin G (IgG) responses against the purified S-layer-like antigen by enzyme-linked immunosorbent assay. IgG responses against this antigen were low in healthy control subjects, but they were significantly higher in subjects with adult and early-onset periodontitis. Together with the fact that the IgG responses against the crude extract of B. forsythusdid not rise significantly in patients with periodontitis, S-layer-like proteins are considered to be specific antigens of B. forsythusand may play an important role in the progression of periodontitis. |
| Databáze: |
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