| Autor: |
Poxleitner, Marianne K., Dawson, Scott C., Cande, W. Zacheus |
| Zdroj: |
Eukaryotic Cell; April 2008, Vol. 7 Issue: 4 p569-574, 6p |
| Abstrakt: |
ABSTRACTGiardia intestinalisis a ubiquitous intestinal protozoan parasite and has been proposed to represent the earliest diverging lineage of extant eukaryotes. Despite the importance of Giardiaas a model organism, research on Giardiahas been hampered by an inability to achieve cell cycle synchrony for in vitro cultures. This report details successful methods for attaining cell cycle synchrony in Giardiacultures. The research presented here demonstrates reversible cell cycle arrest in G1/S and G2/M with aphidicolin and nocodazole, respectively. Following synchronization, cells were able to recover completely from drug treatment and remained viable and maintained synchronous growth for 6 h. These techniques were used to synchronize Giardiacultures to increase the percentages of mitotic spindles in the cultures. This method of synchronization will enhance our ability to study cell cycle-dependent processes in G. intestinalis. |
| Databáze: |
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