| Autor: |
Fagundes, Marcia Regina von Zeska Kress, Semighini, Camile P., Malavazi, Iran, Savoldi, Marcela, de Lima, Joel Fernandes, de Souza Goldman, Maria Helena, Harris, Steven D., Goldman, Gustavo Henrique |
| Zdroj: |
Eukaryotic Cell; July 2005, Vol. 4 Issue: 7 p1239-1252, 14p |
| Abstrakt: |
ABSTRACTThe ATM/ATR kinases and the Mre11 (Mre11-Rad50-Nbs1) protein complex are central players in the cellular DNA damage response. Here we characterize possible interactions between Aspergillus nidulans uvsBATRand the Mre11 complex (scaANBS1). We demonstrate that there is an epistatic relationship between uvsBATR, the homolog of the ATR/MEC1 gene, and scaANBS1, the homolog of the NBS1/XRS2 gene, for both repair and checkpoint functions and that correct ScaANBS1expression during recovery from replication stress depends on uvsBATR. In addition, we also show that the formation of UvsC foci during recovery from replication stress is dependent on both uvsBATRand scaANBS1function. Furthermore, ScaANBS1is also dependent on uvsBATRfor nuclear focus formation upon the induction of DNA double-strand breaks by phleomycin. Our results highlight the extensive genetic interactions between UvsB and the Mre11 complex that are required for S-phase progression and recovery from DNA damage. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
