| Autor: |
Shah, Preetam H., MacFarlane, Ryan C., Bhattacharya, Dhruva, Matese, John C., Demeter, Janos, Stroup, Suzanne E., Singh, Upinder |
| Zdroj: |
Eukaryotic Cell; March 2005, Vol. 4 Issue: 3 p504-515, 12p |
| Abstrakt: |
ABSTRACTVariable phenotypes have been identified for Entamoebaspecies. Entamoeba histolyticais invasive and causes colitis and liver abscesses but only in ~10% of infected individuals; 90% remain asymptomatically colonized. Entamoeba dispar, a closely related species, is avirulent. To determine the extent of genetic diversity among Entamoebaisolates and potential genotype-phenotype correlations, we have developed an E. histolyticagenomic DNA microarray and used it to genotype strains of E. histolyticaand E. dispar. On the basis of the identification of divergent genetic loci, all strains had unique genetic fingerprints. Comparison of divergent genetic regions allowed us to distinguish between E. histolyticaand E. dispar, identify novel genetic regions usable for strain and species typing, and identify a number of genes restricted to virulent strains. Among the four E. histolyticastrains, a strain with attenuated virulence was the most divergent and phylogenetically distinct strain, raising the intriguing possibility that genetic subtypes of E. histolyticamay be partially responsible for the observed variability in clinical outcomes. This microarray-based genotyping assay can readily be applied to the study of E. histolyticaclinical isolates to determine genetic diversity and potential genotypic-phenotypic associations. |
| Databáze: |
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