| Autor: |
Wilkinson, Thomas R., Hall, Elizabeth R. |
| Zdroj: |
Applied Microbiology; January 1971, Vol. 21 Issue: 1 p112-118, 7p |
| Abstrakt: |
Listeria monocytogeneswas successfully isolated from experimentally infected mice by placing homogenized tissues in phosphate-buffered saline (PBS), trypsin, peptone, or pepsin followed by incubation at 37 C for 24 hr. A larger number of Listeriaisolates were recovered from the trypsin or PBS splenic homogenate suspensions incubated at 37 C for 2 hr than from the other diluent suspensions. Holding infected tissues at 4 C for at least 3 months did not increase the efficiency of Listeriaisolation. ListeriaL-forms were not isolated from mice injected with the bacterial form. The in vitro viability of ListeriaL-forms suspended in PBS or PBS splenic homogenate was greatly reduced when held at 4 C. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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