| Autor: |
Hasegawa, T., Pearson, A. M., Price, J. F., Lechowich, R. V. |
| Zdroj: |
Applied Microbiology; July 1970, Vol. 20 Issue: 1 p117-122, 6p |
| Abstrakt: |
Comparisons of the starch-gel patterns of uninoculated aseptic control samples from rabbit and pig muscle with similar samples inoculated and incubated with Clostridium perfringens, Salmonella enteritidis, Achromobacter liquefaciens, and Kurthia zopfiiwere made. Results indicated that C. perfringenscaused extensive alteration in the proteins or enzymes, or both, of the sarcoplasmic fraction of porcine muscle, whereas S. enteritidisand S. faecaliscaused complete breakdown of only myoglobin. Neither A. liquefaciensnor K. zopfiishowed any measurable amount of proteolysis in the sarcoplasmic fraction from pig muscle. Although some of the bands in the starch-gel pattern of rabbit muscle decreased in size and intensity of staining, complete proteolysis of any protein fraction was absent for all test organisms. The disc-gel patterns of the 8 murea-soluble proteins showed that C. perfringenscaused extensive proteolysis in pig muscle and a lesser extent of proteolysis in rabbit muscle. None of the other organisms utilized in this study had any measurable effect upon the urea-soluble proteins. In addition, a simple procedure for aseptic isolation of muscle samples for studying meat spoilage is outlined. Results indicate that careful sanitation and cleanliness will give suitable samples for meat spoilage investigations. |
| Databáze: |
Supplemental Index |
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