The Mass Culture of Porphyridium cruentum

Autor: Golueke, Clarence G., Oswald, William J.
Zdroj: Applied Microbiology; March 1962, Vol. 10 Issue: 2 p102-107, 6p
Abstrakt: A study was made of the effect of temperature, detention period, light intensity, and salinity on the growth rate and over-all light energy conversion efficiency of Porphyridium cruentumcultured on a medium consisting of concentrated sea water and sewage enriched with urea, chelated iron, and other additives. It was found that the optimal temperature was within the range of 21 to 26 C. Growth was retarded at temperatures less than 13 C, and completely inhibited above 31 C. Over-all light energy conversion efficiency increased from 2.24% at the 4-day detention period to 2.76% at the 10-day period. Conversion efficiency ranged from 5.8% at a light energy absorption rate of 8.2 cal:liter:min to 2.3% at 35 to 39 cal:liter:min.At salt concentrations less than 3.5%, Porphyridiumcould not successfully compete with other algae in open cultures. Salt concentrations as high as 4.6% had no inhibitory effect on its growth.In studies on nutrition, it was found that growth on a medium of salts used in formulating synthetic sea water dissolved in sewage was equal to that on a control medium consisting of concentrated sea water and sewage (see above). They showed that sewage contains a substance or substances essential for optimal growth. Vitamin B12alone could not substitute for it.
Databáze: Supplemental Index