| Autor: |
De Dios Andres, Paula, Westensee, Isabella N., Brodszkij, Edit, Ramos-Docampo, Miguel A., Gal, Noga, Städler, Brigitte |
| Zdroj: |
Biomacromolecules; September 2021, Vol. 22 Issue: 9 p3860-3872, 13p |
| Abstrakt: |
Cell culture-based intestinal models are important to evaluate nanoformulations intended for oral drug delivery. We report the use of a floating structured paper chip as a scaffold for Caco-2 cells and HT29-MTX-E12 cells that are two established cell types used in intestinal cell models. The formation of cell monolayers for both mono- and cocultures in the paper chip are confirmed and the level of formed cell–cell junctions is evaluated. Further, cocultures show first mucus formation between 6–10 days with the mucus becoming more pronounced after 19 days. Hybrid vesicles (HVs) made from phospholipids and the amphiphilic block copolymer poly(cholesteryl methacrylate)-block-poly(2-carboxyethyl acrylate) in different ratios are used as a representative soft nanoparticle to assess their mucopenetration ability in paper chip-based cell cultures. The HV assembly is characterized, and it is illustrated that these HVs cross the mucus layer and are found intracellularly within 3 h when the cells are grown in the paper chips. Taken together, the moist three-dimensional cellulose environment of structured paper chips offers an interesting cell culture-based intestinal model that can be further integrated with fluidic systems or online read-out opportunities. |
| Databáze: |
Supplemental Index |
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