| Autor: |
Auletta, Angela E., Gitnick, Gary L., Whitmire, Carrie E., Sever, John L. |
| Zdroj: |
Applied Microbiology; May 1968, Vol. 16 Issue: 5 p691-694, 4p |
| Abstrakt: |
Rubella hemagglutinating (HA) antigen was prepared in BHK-21 tissue as 5% cell suspensions and from unconcentrated and 20× concentrated infected supernatant fluids. In some instances, unconcentrated fluids were treated with Tween 80 and ether; cell suspensions were treated with ether alone. Preparations were tested for HA activity in dextrose-gelatin-Veronal (DGV) buffer solutions; 0.85% NaCl; Sorenson's phosphate-buffered saline, pH 7.2; and a diluent of 0.9% NaCl, 0.1% CaCl2(anhydrous), and 0.1% MgSO4·7H2O. HA titers were consistently two- to fourfold higher in the saline with added Ca++and Mg++than in DGV. Hemagglutination-inhibition titers of paired human sera were the same in either diluent. It is suggested that the interaction between rubella HA antigen and the red cells of young (less than 1-day-old) chicks may be at least partially ion dependent and that titers are enhanced by increased quantities of divalent cations. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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