| Autor: |
Sokol, P A, Iglewski, B H, Hager, T A, Sadoff, J C, Cross, A S, McManus, A, Farber, B F, Iglewski, W J |
| Zdroj: |
Infection and Immunity; October 1981, Vol. 34 Issue: 1 p147-153, 7p |
| Abstrakt: |
Exoenzyme S differs from toxin A and diphtheria toxin in that it does not adenosine diphosphate (ADP)-ribosylate elongation factor-2, but rather catalyzes the transfer of the ADP-ribose moiety of nicotinamide adenine dinucleotide to a number of different proteins in extracts of eucaryotic cells. Polyoma-transformed BHK-21 cells were isolated which were resistant to diphtheria toxin and toxin A. Extracts from these cells are ADP-ribosylated by exoenzyme S but not toxin A or diphtheria toxin, providing an assay which distinguishes between S and A activities. A total of 124 clinical isolates of P. aeruginosa were analyzed for production of toxin A and exoenzyme S. Exoenzyme S production was detected in 38% of the strains, whereas 80% of the strains produced toxin A. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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