| Autor: |
Ansah, Felix, Suurbaar, Jonathan, Darko, Derrick, Anabire, Nsoh Godwin, Blankson, Samuel O., Domson, Bright K.S., Soulama, Alamissa, Kpasra, Paulina, Chirawurah, Jersley D., Amenga-Etego, Lucas, Kanyong, Prosper, Awandare, Gordon A., Aniweh, Yaw |
| Zdroj: |
The Journal of Molecular Diagnostics; 20210101, Issue: Preprints |
| Abstrakt: |
Plasmodium malariaeand Plasmodium ovaleare increasingly gaining public health attention as the global transmission of falciparum malaria is decreasing. However, the absence of reliable Plasmodiumspecies-specific detection tools has hampered accurate diagnosis of these “minor” Plasmodiumspecies. In this study, SYBR Green-based real-time PCR assays were developed for the detection of P. malariaeand P. ovaleusing cooperative primers that significantly limit the formation and propagation of primer-dimers. Both the P. malariaeand P. ovalecooperative primer-based assays had at least 10-fold lower detection limit compared to the corresponding conventional primers. More importantly, the cooperative primer-based assays were evaluated in a cross-sectional study using 560 samples obtained from two health facilities in Ghana. The prevalence of P. malariaeand P. ovaleamong the combined study population were 18.6% (104/560) and 5.5% (31/560), respectively. Among the Plasmodiumpositive cases, P. malariaeand P. ovalemono-infections were 3.6% (18/499) and 1.0% (5/499), respectively, with the remaining being co-infections with P. falciparum. The study demonstrates the public health importance of including detection tools with lower detection limits in routine diagnosis and surveillance of non-falciparum species. This will be necessary for comprehensively assessing the effectiveness of malaria interventions and control measures aimed towards global malaria elimination. |
| Databáze: |
Supplemental Index |
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