Pyramiding Ty-1/Ty-3, Ty-2, ty-5 and ty-6genes into tomato hybrid to develop resistance against tomato leaf curl viruses and recurrent parent genome recovery by ddRAD sequencing method

Autor: Prabhandakavi, Pardhasaradhi, Pogiri, Rambabu, Kumar, Rakesh, Acharya, Santanu, Esakky, Ramchandran, Chakraborty, M., Pinnamaneni, Rajasekhar, Palicherla, Sairam Reddy
Zdroj: Journal of Plant Biochemistry and Biotechnology; September 2021, Vol. 30 Issue: 3 p462-476, 15p
Abstrakt: Tomato leaf curl virus (ToLCV) is a begomovirus transmitted by whitefly (Bemisia tabaci) and causes Tomato (yellow) leaf curl disease (TYLCD/ToLCD) in many parts of India and rest of the world resulting in huge economic losses. Many Tygenes identified either from wild and/or cultivated tomato, which confers various levels of resistance to ToLCV. The current study aimed at stacking four important resistant genes (Ty-1/Ty-3, Ty-2, ty-5,andty-6) in parental lines of a commercial tomato hybrid JKTH811 with the aid of the marker-assisted backcross breeding program. As a donor source, two pre-breeding lines, CLN3125K (Ty-1/Ty-3andTy-2) and FLA456 (ty-5and ty-6) was used. Crossed population screened by using tightly linked Tygenes specific markers for foreground selection and attempted the fastest recovery of recurrent parental genome through NGS based ddRAD sequencing approach. Tygenes introgressed parental lines as well as the resulting F1hybrids were tested for their bio-efficacy against ToLCNDV (New Delhi var.) under greenhouse and field conditions. Disease severity of Tygenes pyramided female lines and F1hybrids were found as significantly lower when compared to original parental lines and hybrid. This report demonstrates the efficiency of Tygenes pyramiding from different donors in a hybrid against ToLCV and recovery of the recurrent genome using the ddRAD-seq platform in the minimum generations possible.
Databáze: Supplemental Index
Externí odkaz: