| Autor: |
Zhang, Bin, Qiu, Liyao, Long, Chaoying, Gao, Zhihua |
| Zdroj: |
STAR Protocols; 20210101, Issue: Preprints |
| Abstrakt: |
The hypothalamic magnocellular neuroendocrine cells (MNCs) project to the posterior pituitary (PPi), regulating reproduction and fluid homeostasis. It has been challenging to selectively label and manipulate MNCs, as they are intermingled with parvocellular neuroendocrine cells projecting to the median eminence. Here, we provide a step-by-step protocol for specifically targeting the MNCs by infusing retrograde viral tracers into the PPi. When combined with optogenetics, chemogenetics, and transgenic animals, this approach allows cell-type-specific manipulation of MNCs in multiple sites for functional dissection. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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