Autor: |
Economou, J S, Rhoades, K, Essner, R, McBride, W H, Gasson, J C, Morton, D L |
Zdroj: |
The Journal of Experimental Medicine; July 1989, Vol. 170 Issue: 1 p321-326, 6p |
Abstrakt: |
The 615-bp 5' flanking region of the human TNF-alpha/cachectin gene was isolated and ligated to the luciferase reporter gene. In addition, a series of truncated promoter constructs was generated by exonuclease III digestion. The promoter activity of these constructs was studied in a transient transfection system using the TNF-alpha-producing U937 cell line. Full-length and truncated TNF promoter constructions extending from -615 to -95 bp relative to the transcription start site (TSS) could be induced by phorbol esters. A construct truncated to within 36 bp of the TSS (and within 11 bp of the TATAA box) was inactive. Therefore, the phorbol ester responsive is localized in the TNF/cachectin promoter to a relatively short region proximal to the TATAA box. |
Databáze: |
Supplemental Index |
Externí odkaz: |
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