| Autor: |
Labéta, Mario O., Vidal, Karine, Nores, Julia E. Rey, Arias, Mauricio, Vita, Natalio, Morgan, B. Paul, Guillemot, Jean Claude, Loyaux, Denis, Ferrara, Pascual, Schmid, Daniel, Affolter, Michael, Borysiewicz, Leszek K., Donnet-Hughes, Anne, Schiffrin, Eduardo J. |
| Zdroj: |
The Journal of Experimental Medicine; May 2000, Vol. 191 Issue: 10 p1807-1812, 6p |
| Abstrakt: |
Little is known about innate immunity to bacteria after birth in the hitherto sterile fetal intestine. Breast-feeding has long been associated with a lower incidence of gastrointestinal infections and inflammatory and allergic diseases. We found in human breast milk a 48-kD polypeptide, which we confirmed by mass spectrometry and sequencing to be a soluble form of the bacterial pattern recognition receptor CD14 (sCD14). Milk sCD14 (m-sCD14) concentrations were up to 20-fold higher than serum sCD14 from nonpregnant, pregnant, or lactating women. In contrast, lipopolysaccharide (LPS)-binding protein was at very low levels. Mammary epithelial cells produced 48-kD sCD14. m-sCD14 mediated activation by LPS and whole bacteria of CD14 negative cells, including intestinal epithelial cells, resulting in release of innate immune response molecules. m-sCD14 was undetectable in the infant formulas and commercial (cows') milk tested, although it was present in bovine colostrum. These findings indicate a sentinel role for sCD14 in human milk during bacterial colonization of the gut, and suggest that m-sCD14 may be involved in modulating local innate and adaptive immune responses, thus controlling homeostasis in the neonatal intestine. |
| Databáze: |
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