| Autor: |
Darsley, M J, Takahashi, H, Macchi, M J, Frelinger, J A, Ozato, K, Appella, E |
| Zdroj: |
The Journal of Experimental Medicine; January 1987, Vol. 165 Issue: 1 p211-222, 12p |
| Abstrakt: |
The specificities of an extensive panel of anti-H-2Dd monoclonal antibodies, which had been previously characterized using exon-shuffled H-2Dd/H-2Ld molecules and a number of anti-H-2DP antibodies, were examined using H-2Dd/H-2DP recombinants. The use of this new family of recombinant antigens revealed extensive interaction between the membrane-distal (N and Cl) domains of class I molecules. 20 out of 48 mAbs recognize complex epitopes formed by the interaction of these two domains. These antibodies exhibit a number of distinct patterns of crossreactivity with other class I proteins, revealing the presence of multiple epitopes within the region of domain interaction. Comparison of the data presented here with those from previous work allowed the identification of a small number of residues in the Cl domain that participate in the generation of complex epitopes involving both the N and Cl domains. The results are discussed in terms of the structural information available for these two domains. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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