Label-free microscopy of mitotic chromosomes using the polarization orthogonality breaking technique

Autor: Desapogu, Rajesh, Le Marchand, Gilles, Smith, Rebecca, Ray, Paulami, Gillier, Émilie, Dutertre, Stéphanie, Alouini, Mehdi, Tramier, Marc, Huet, Sébastien, Fade, Julien
Zdroj: Biomedical Optics Express; August 2021, Vol. 12 Issue: 8 p5290-5304, 15p
Abstrakt: We report how a recently developed polarization imaging technique, implementing micro-wave photonics and referred to as orthogonality-breaking (OB) imaging, can be adapted on a classical confocal fluorescence microscope, and is able to provide informative polarization images from a single scan of the cell sample. For instance, the comparison of the images of various cell lines at different cell-cycle stages obtained by OB polarization microscopy and fluorescence confocal images shows that an endogenous polarimetric contrast arizes with this instrument on compacted chromosomes during cell division.
Databáze: Supplemental Index