| Autor: |
Hongo, Sadato, Okada, Jun, Hashimoto, Koji, Tsuji, Koichi, Nikaido, Masaru, Gemma, Nobuhiro |
| Zdroj: |
SICE Journal of Control, Measurement, and System Integration; May 2008, Vol. 1 Issue: 3 p265-270, 6p |
| Abstrakt: |
A new compact automated DNA detection system Genelyzer™ has been developed. After injecting a sample solution into a cassette with a built-in electrochemical DNA chip, processes from hybridization reaction to detection and analysis are all operated fully automatically. In order to detect a sample DNA, electrical currents from electrodes due to an oxidization reaction of electrochemically active intercalator molecules bound to hybridized DNAs are detected. The intercalator is supplied as a reagent solution by a fluid supply unit of the system. The feasibility test proved that the simultaneous typing of six single nucleotide polymorphisms (SNPs) associated with a rheumatoid arthritis (RA) was carried out within two hours and that all the results were consistent with those by conventional typing methods. It is expected that this system opens a new way to a DNA testing such as a test for infectious diseases, a personalized medicine, a food inspection, a forensic application and any other applications. |
| Databáze: |
Supplemental Index |
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