| Autor: |
Magi, Gloria, Capretti, Roberta, Paoletti, Claudia, Pietrella, Marco, Ferrante, Luigi, Biavasco, Francesca, Varaldo, Pietro Emanuele, Facinelli, Bruna |
| Zdroj: |
Antimicrobial Agents and Chemotherapy; May 2003, Vol. 47 Issue: 5 p1571-1576, 6p |
| Abstrakt: |
ABSTRACTSex pheromone plasmids, frequently found in Enterococcus faecalis, have rarely been detected in Enterococcus faecium. pBRG1 is an approximately 50-kb vanA-carrying conjugative plasmid of an E. faeciumclinical isolate (LS10) that is transferable to E. faecalislaboratory strains. In cell infection experiments, E. faeciumLS10 exhibited remarkably high invasion efficiency and produced cytopathogenic effects in Caco-2 cell monolayers. Growth in the presence of sex pheromones produced by E. faecalisJH2-2 was found to cause self-aggregation of both E. faeciumLS10 and E. faecalisJH-RFV(pBRG1) (a transconjugant obtained by transfer of pBRG1 to E. faecalisJH2-2) and to increase the cell adhesion and invasion efficiencies of both E. faeciumLS10 and E. faecalisJH-RFV(pBRG1). Sex pheromone cCF10 caused clumping of E. faecalisOG1RF(pBRG1) (a transconjugant obtained by transfer of pBRG1 to E. faecalisOG1RF) at a concentration ∼100-fold higher than the one required for the control strain E. faecalisOG1RF(pCF10). PCR products of the expected sizes were obtained with primers internal to aggregation substance genes of E. faecalispheromone response plasmids pAD1, pPD1, and pCF10 and primers internal to ash701of E. faeciumpheromone plasmid pHKK701. These findings suggest that pBRG1 of E. faeciumLS10 is a sex pheromone response plasmid. |
| Databáze: |
Supplemental Index |
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