| Autor: |
Lozniewski, Alain, Duprez, Adrien, Renault, Corinne, Muhale, Filipe, Conroy, Marie-Christine, Weber, Michele, Le Faou, Alain, Jehl, Francois |
| Zdroj: |
Antimicrobial Agents and Chemotherapy; August 1999, Vol. 43 Issue: 8 p1909-1913, 5p |
| Abstrakt: |
ABSTRACTThe delivery of antibiotics into Helicobacter pylori-infected human stomachs is still poorly understood. Human embryonic gastric xenografts in nude mice have recently been proposed as a new model for the study of H. pyloriinfection. Using this model, we compared the penetration of amoxicillin, after intraperitoneal administration of a dose of 20 mg/kg of body weight, into the gastric mucosae of infected and uninfected xenografts. The concentrations of this drug in serum and superficial gastric mucosae were determined at 20 min and 1 and 3 h after injection. Ten mice with H. pylori-infected grafts (n= 5) or uninfected grafts (n= 5) were studied. Mucosal samples were obtained by cryomicrotomy. The concentrations in serum were similar to those obtained in the serum of humans after oral administration of 1 g of amoxicillin. The mean area under the tissue concentration-versus-time curve from 0 to 3 h obtained for mice with infected grafts was significantly higher than that obtained for the animals with uninfected grafts (P= 0.01). These results suggest that the penetration of amoxicillin into the superficial gastric mucosa may be substantially increased in the case of H. pyloriinfection. Thus, human xenografts in nude mice represent a new, well-standardized model for investigation of systemic delivery of drugs into H. pylori-infected gastric mucosa. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
