| Autor: |
Saeland, Sem, Duvert, Valerie, Pandrau, Dominique, Caux, Christophe, Durand, Isabelle, Wrighton, Nicholas, Wideman, Janusz, Lee, Frank, Banchereau, Jacques |
| Zdroj: |
Blood; November 1991, Vol. 78 Issue: 9 p2229-2238, 10p |
| Abstrakt: |
In the present study, we investigated the effects of human recombinant interleukin-7 (IL-7) on the proliferation of enriched hematopoietic cells isolated from human adult and fetal bone marrow (BM). In cultures of CD34+cells, IL-7 was found to induce dose-dependent incorporation of 3H-thymi-dine pH-TdR), but had no demonstrable effect on the development of myeloid colony-forming cells. Numbers of B-cell precursors (BCP), initially present within CD34+populations and which included a CD34 CD20+subset, were significantly increased when CD34+BM cells were cultured in the presence of IL-7. This effect was most striking on CD20+BCP, and resulted at least partly from higher numbers of cycling cells as indicated by Hoechst 33342 fluorescence (Calbiochem, Behring Diagnostics, La Jolla, CA). These results indicate that IL-7 promotes the growth of BCP within the CD34+ compartment. In line with the B-lineage affiliation of CD34+ target cells, committed BCP (CD10+ CD19+ surface IgM) isolated from BM were also found to proliferate in response to IL-7. Interestingly, this effect of IL-7 was strongly potentiated by the addition of IL-3. Taken together, and in accordance with previous observations on murine cells, our data indicate that IL-7 acts as a growth factor during the ontogeny of human B lymphocytes.© 1991 by The American Society of Hematology. 0006-4971/91 / 7809-0003$3.00/0 |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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