| Autor: |
Reijden, Bert A. van der, Lombardo, Mariella, Dauwerse, Hans G., Giles, Rachel H., Mühlematter, Dominique, Bellomo, Martine Jotterand, Wessels, Hans W., Beverstock, Geoff C., Ommen, Gert-Jan B. van, Hagemeijer, Anne, Breuning, Martijn H. |
| Zdroj: |
Blood; July 1995, Vol. 86 Issue: 1 p277-282, 6p |
| Abstrakt: |
As acute nonlymphocytic leukemia (ANLL) with inv(16) (p13q22) or t(16; 16)(p13;q22) has been shown to result from the fusion of transcription factor subunit core binding factor (CBFB) to a myosin heavy chain (MYH11), we sought to design methods to detect this rearrangement using reverse transcriptase-polymerase chain reaction (RT-PCR). In all of 27 inv(16)(p13q22) and four t(16;16)(p13;q22) cases tested, a chimeric CBFB-MYH11 transcript coding for an in-frame fusion protein was detected. In a more extensive RT-PCR analysis with different primer pairs, we detected a second new chimeric CBFB-MYH11 transcript in 10 of 11 patients tested. The CBFB-MYH11 reading frame of the second transcript was maintained in one patient but not in the others. We show that the different CBFB-MYH11 transcripts in one patient arise from alternative splicing. Translation of the transcript in which the CBFB-MYH11 reading frame is not maintained leads to a slightly truncated CBFB protein. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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