| Autor: |
Bodger, Michael P., Janossy, George, Bollum, Fred J., Burford, Graham D., Hoffbrand, A. Victor |
| Zdroj: |
Blood; June 1983, Vol. 61 Issue: 6 p1125-1131, 7p |
| Abstrakt: |
The ontogeny of cells containing the enzyme terminal deoxynucleotidyl transferase (TdT) in human fetal liver, bone marrow, and thymus has been studied using a highly specific antiserum to TdT together with monoclonal anti-precursor cell antibodies in double and triple marker immunofluorescence. TdT+cells were first observed in fetal liver at 12 wk of gestation and accounted for 55% of the lymphoid-like cells isolated after Ficoll-Hypaque separation. TdT4cells were first observed in the bone marrow 16 wk after gestation. Like TdT+cells in normal infant bone marrow, the majority of TdT+cells in fetal liver and bone marrow expressed both BA-1 and RFB-1 antigens. This suggests that fetal TdT+cells include progenitors of the B lineage (BA-1+) and perhaps of thymocytes (RFB-1+). Nevertheless, TdT was not observed in fetal thymocytes until after 20 wk of gestation, although thymic blasts and the majority of thymocytes were strongly RFB-1+from 12 wk of gestation. These results clearly show that fetal thymus is first populated by TdT-, RFB-1+, BA-1-cells, but does not exclude the fact that a second "wave" of TdT4prothymocytes, possibly bone marrow derived, also exists. |
| Databáze: |
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