| Autor: |
Klechevsky, Eynav, Flamar, Anne-Laure, Cao, Yanying, Blanck, Jean-Philippe, Liu, Maochang, O'Bar, Amy, Agouna-Deciat, Olivier, Klucar, Peter, Thompson-Snipes, LuAnn, Zurawski, Sandra, Reiter, Yoram, Palucka, A. Karolina, Zurawski, Gerard, Banchereau, Jacques |
| Zdroj: |
Blood; September 2010, Vol. 116 Issue: 10 p1685-1697, 13p |
| Abstrakt: |
We evaluated human CD8+T-cell responses generated by targeting antigens to dendritic cells (DCs) through various lectin receptors. We found the immunoreceptor tyrosine-based inhibitory motif-containing DC immunoreceptor (DCIR) to mediate potent cross-presentation. A single exposure to a low dose of anti-DCIR–antigen conjugate initiated antigen-specific CD8+T-cell immunity by all human DC subsets including ex vivo–generated DCs, skin-isolated Langerhans cells, and blood myeloid DCs and plasmacytoid DCs. The delivery of influenza matrix protein (FluMP) through DCIR resulted in expansion of FluMP-specific memory CD8+T cells. Enhanced specific CD8+T-cell responses were observed when an antigen was delivered to the DCs via DCIR, compared with those induced by a free antigen, or antigen conjugated to a control monoclonal antibody or delivered via DC-SIGN, another lectin receptor. DCIR targeting also induced primary CD8+T-cell responses against self (MART-1) and viral (HIV gag) antigens. Addition of Toll-like receptor (TLR) 7/8 agonist enhanced DCIR-mediated cross-presentation as well as cross-priming, particularly when combined with a CD40 signal. TLR7/8 activation was associated with increased expansion of the primed CD8+T cells, high production of interferon-γ and tumor necrosis factor-α, and reduced levels of type 2–associated cytokines. Thus, antigen targeting via the human DCIR receptor allows activation of specific CD8+T-cell immunity. |
| Databáze: |
Supplemental Index |
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