| Autor: |
de Lima Silva, Lucas Luiz, Oliveira, Ana Karoline Silva, Gama, Aline Rodrigues, Ramos, Amanda Ferreira Paes Landim, Silva, Antonio Márcio Teodoro Cordeiro, Blanco, Angel José Vieira, Vieira, José Daniel Gonçalves, Rasmussem, Lucas Trevizani, Carneiro, Lilian Carla, Barbosa, Mônica Santiago |
| Zdroj: |
Brazilian Journal of Microbiology; December 2021, Vol. 52 Issue: 4 p1921-1927, 7p |
| Abstrakt: |
Helicobacter pyloriis the etiological agent of chronic gastritis, peptic ulcer, and gastric cancer. The duodenal ulcer-promoting gene dupA, which is located in the plasticity region of the H. pylorigenome, is homologous to the virBgene which encodes a type IV secretion protein in Agrobacterium tumefaciens. Studies have shown associations between H. pylori dupA-positive strains and gastroduodenal diseases. However, whether dupAacts as a risk factor or protective factor in these diseases remains unclear. Therefore, in this study, we aimed to verify the presence of the dupAgene in infectious H. pyloristrains in the Brazilian mid-west and to investigate its association with the clinical outcomes of patients with dyspepsia. Additionally, the phylogenetic origin of the strains was determined. Gastric biopsies from 117 patients with dyspepsia were analyzed using histological and molecular techniques. The hpxgene (16S rRNA) was used to screen for H. pyloriinfection, and positive samples were then subjected to dupAgene detection and sequencing. The estimated prevalence of H. pyloriinfection was 64.1%, with the dupAgene being detected in a high proportion of infectious strains (70.7%). Furthermore, a risk analysis revealed that for women, a dupA-positive H. pyloriinfection increased the chance of developing gastritis by twofold. The partial dupAsequences from isolated infectious strains in this work are similar to those of strains isolated in westerns countries. This study provides useful insights for understanding the role of the H. pylori dupAgene in disease development. |
| Databáze: |
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