Effect of Ribosomal Wash Factors on Inhibition by Chloramphenicol

Autor: Cameron, Helen J., Rogers, Samuel J., Julian, Gordon R.
Zdroj: Antimicrobial Agents and Chemotherapy; September 1972, Vol. 2 Issue: 3 p156-158, 3p
Abstrakt: In vitro protein-synthesizing systems from Escherichia colican be categorized as either chloramphenicol-sensitive or chloramphenicol-insensitive. The chloramphenicol-sensitive systems used in this study required the presence of factors removed from ribosomes with 1.0 mNH4Cl when chromatographically purified ribosomes were used for amino acid incorporation. These ribosomal wash factors inhibited but did not eliminate amino acid incorporation in chloramphenicol-insensitive systems. For both systems, addition of increasing amounts of the ribosomal wash factors increased the sensitivity to chloramphenicol inhibition.
Databáze: Supplemental Index