| Autor: |
Stolla, Massiel Chavez, Catherman, Seana C., Kingsley, Paul D., Rowe, R. Grant, Koniski, Anne D., Fegan, Katherine, Vit, Leah, McGrath, Kathleen E., Daley, George Q., Palis, James |
| Zdroj: |
Blood Advances; January 2019, Vol. 3 Issue: 1 p72-82, 11p |
| Abstrakt: |
Platelets are essential for hemostasis; however, several studies have identified age-dependent differences in platelet function. To better understand the origins of fetal platelet function, we have evaluated the contribution of the fetal-specific RNA binding protein Lin28bin the megakaryocyte/platelet lineage. Because activated fetal platelets have very low levels of P-selectin, we hypothesized that the expression of platelet P-selectin is part of a fetal-specific hematopoietic program conferred by Lin28b. Using the mouse as a model, we find that activated fetal platelets have low levels of P-selectin and do not readily associate with granulocytes in vitro and in vivo, relative to adult controls. Transcriptional analysis revealed high levels of Lin28band Hmga2in fetal, but not adult, megakaryocytes. Overexpression of LIN28Bin adult mice significantly reduces the expression of P-selectin in platelets, and therefore identifies Lin28bas a negative regulator of P-selectin expression. Transplantation of fetal hematopoietic progenitors resulted in the production of platelets with low levels of P-selectin, suggesting that the developmental regulation of P-selectin is intrinsic and independent of differences between fetal and adult microenvironments. Last, we observe that the upregulation of P-selectin expression occurs postnatally, and the temporal kinetics of this upregulation are recapitulated by transplantation of fetal hematopoietic stem and progenitor cells into adult recipients. Taken together, these studies identify Lin28bas a new intrinsic regulator of fetal platelet function. |
| Databáze: |
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