| Autor: |
Dokter, Willem H.A., Esselink, Mariet T., Halie, M. Ruud, Vellenga, Edo |
| Zdroj: |
Blood; January 1993, Vol. 81 Issue: 2 p337-343, 7p |
| Abstrakt: |
We studied the effect of interleukin-4 (IL-4) on the lipopolysaccharide (LPS) induction of two immediate early genes c-fosand c-jun.These genes encode proteins that form the dimeric complex activator protein-1 (AP-1), which is active as a transcriptional factor. Maximal accumulation of either c-fosand c-junmessenger RNA (mRNA) occurred 30 minutes after LPS addition. When cells were treated with IL-4 for 5 hours before LPS activation, both the c-fosand the c-junmRNA expression was decreased. The inhibition of c-fosand c-junexpression by IL-4 in LPS-treated cells was shown to be due to a lower transcription rate of the c-fosand c-jungenes. IL-4 did not affect the stability of the c-fosand c-juntranscripts. Finally, using electrophoretic mobility shift assays, evidence was obtained that IL-4 inhibits LPS-induced expression of AP-1 protein. These data indicate that IL-4 suppresses the induction of transcription factors in human activated monocytes. |
| Databáze: |
Supplemental Index |
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