| Autor: |
Macdonald, Donald J., Boyle, Rose M., Glen, Alastair C.A., Horrobin, David F. |
| Zdroj: |
Blood; May 2004, Vol. 103 Issue: 9 p3562-3564, 3p |
| Abstrakt: |
Phospholipase A2type IVA (IVAPLA2) is a cytosolic enzyme that on activation selectively releases arachidonic acid (AA) from cell membrane phospholipids. Both AA and lysophospholipid, products of the enzymic reaction, can function as signal transducers in cellular interactions. The enzyme is present in most cells, including polymorphs, eosinophils, and platelets. This study used affinity purification to extract IVAPLA2from red cell lysate prepared from leukocyte- and platelet-depleted human blood to overcome the masking effect of hemoglobin on Western blot detection. We show that IVAPLA2is present in red cells as a 90-kDa protein. (Blood. 2004;103:3562-3564) |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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