| Autor: |
Fibbe, Willem E., Damme, Jo Van, Billiau, Alfons, Duinkerken, Nelleke, Lurvink, Elly, Ralph, Peter, Altrock, Bruce W., Kaushansky, Kenneth, Willemze, Roel, Falkenburg, J.H. Frederik |
| Zdroj: |
Blood; September 1988, Vol. 72 Issue: 3 p860-866, 7p |
| Abstrakt: |
Electrophoretically pure human interleukin-1 (IL-1) beta was found to stimulate human fibroblasts in a monolayer culture to elaborate colony-stimulating activity (CSA). Supernatant fluids from cultures induced with increasing concentrations of IL-1 were found to stimulate colony formation of myeloid (CFU-GM), erythroid (BFU-E), and multipotent (CFU-GEMM) progenitor cells in a dose-depen-dent fashion. The effect on mixed colony formation, however, was less than on CFU-GM and BFU-E growth. Similar to IL-1, the synthetical double-stranded RNA poly(rl).poly(rC) also stimulated release of CSA by fibroblasts. The kinetics of IL-1- and poly(rl).poly(rC)-induced CSA release were found to be different, in that poly(rl).poly(rC)-induced CSA production occurred more slowly. Anti-IL-1 antiserum was able to completely neutralize the IL-1 -induced CSA release, but had no effect on poly(rl).poly(rC)-induced CSF production, suggesting that the latter effect was mediated by other mechanisms than IL-1 in supernatant. By the use of specific immunologic assays, G-CSF, M-CSF, and GM-CSF could be identified in media conditioned by fibroblasts treated with IL-1 or poly(rl).poly(rC). Poly(rl).poly(rC) appeared to be a better inducer for M-CSF than IL-1.1988 by Grune & Stratton, Inc. 0006-4971/88/7203-0001$3.00/0. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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