Histones Induce Endothelial Von Willebrand Factor Release and Subsequent Platelet Capture in In Vitroand In VivoModels

Autor: Michels, Alison, Swystun, Laura L, Albánez, Silvia, Mewburn, Jeffrey, Sponagle, Kate, Lillicrap, David
Zdroj: Blood; December 2014, Vol. 124 Issue: 21 p2768-2768, 1p
Abstrakt: Thrombosis is the leading cause of mortality worldwide and can result from uncontrolled immune activation in inflammatory conditions. Von Willebrand factor (VWF) is an acute phase glycoprotein that mediates primary hemostasis by binding to platelets. It is stored in platelets and in Weibel-Palade bodies (WPB) in endothelial cells. Cell-free DNA (CF-DNA) and histones are released during cell damage and have been shown to stimulate innate immune and pro-thrombotic responses including, in the case of histones, thrombocytopenia in mice and VWF release due to platelet activation/aggregation. We recently demonstrated the correlation between VWF and CF-DNA plasma levels in human and mouse models of chronic inflammation associated with aging. In these studies we investigate the influence of CF-DNA and histones on VWF release and platelet-binding in murine and endothelial cell models.
Databáze: Supplemental Index