The Sea Urchin sns5Chromatin Insulator Improves the Likelihood of Lentiviral Vectors in Erythroid Milieu By Organizing an Independent Chromatin Domain at the Integration Site

Autor: Baiamonte, Elena, Di Stefano, Rosalia, Lo Iacono, Melania, Spina, Barbara, Vitrano, Angela, Di Maggio, Rosario, Sacco, Massimiliano, Spinelli, Giovanni, Maggio, Aurelio, Acuto, Santina, Cavalieri, Vincenzo
Zdroj: Blood; December 2015, Vol. 126 Issue: 23 p4414-4414, 1p
Abstrakt: Retroviral vectors are currently the most suitable vehicles for therapeutic gene transfer in hematopoietic stem cells. However, these vectors are known to integrate rather randomly throughout the genome, suffering the so called chromosomal position effects (PE). Such a critical occurrence most probably depends upon the ability of heterochromatin to spread in the inserted vector sequences. Moreover, the use of transgenes imply genotoxicity effects, since the cis-regulatory sequences harbored by the vector can disturb the proper transcription of the resident genes neighboring the integration site, potentially leading to malignant transformation.
Databáze: Supplemental Index