| Autor: |
Tsai, Che-Chung, Emau, Peter, Jiang, Yonghou, Agy, Michael B., Shattock, Robin J., Schmidt, Ann, Morton, William R., Gustafson, Kirk R., Boyd, Michael R. |
| Zdroj: |
AIDS Research and Human Retroviruses; January 01, 2004, Vol. 20 Issue: 1 p11-18, 8p |
| Abstrakt: |
The cyanobacterial protein cyanovirin-N (CV-N) potently inactivates diverse strains of HIV-1 and other lentiviruses due to irreversible binding of CV-N to the viral envelope glycoprotein gp120. In this study, we show that recombinant CV-N effectively blocks HIV-1Ba-L infection of human ectocervical explants. Furthermore, we demonstrate the in vivo efficacy of CV-N gel in a vaginal challenge model by exposing CV-N-treated female macaques (Macaca fascicularis) to a pathogenic chimeric SIV/HIV-1 virus, SHIV89.6P. All of the placebo-treated and untreated control macaques (8 of 8) became infected. In contrast, 15 of 18 CV-N-treated macaques showed no evidence of SHIV infection. Further, CV-N produced no cytotoxic or clinical adverse effects in either the in vitro or in vivo model systems. Together these studies suggest that CV-N is a good candidate for testing in humans as an anti-HIV topical microbicide. |
| Databáze: |
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